| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Cytomegalovirus DNA, Quantitative Real-Time PCR (QNCMV)
Test CodeLAB2222
Quest Code
10600
CPT Codes
87497
Preferred Specimen
1 mL whole blood or plasma collected in an EDTA (lavender- top) or ACD (yellow-top) tube or 1 mL plasma collected in EDTA PPT (white-top) tube or 1 mL serum collected in a red top tube (no gel) or serum separator tube (SST) or 1 mL CSF or amniotic fluid collected in a sterile leak-proof container.
Minimum Volume
0.5 mL
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF or amniotic fluid: Collect in a sterile plastic leak-proof container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile plastic screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or serum separator tube (SST) and store refrigerated or frozen.
CSF or amniotic fluid: Collect in a sterile plastic leak-proof container and store refrigerated or frozen.
Transport Container
See Collection Instructions
Transport Temperature
Refrigerated; random urine should be frozen.
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen whole blood • Bronchial, nasopharyngeal, or tracheal lavage/wash • Synovial, pericardial, peritoneal, pleural or vitreous fluid • Sputum • Eye • Lesion (vesicle) aspirate • Nasal/nasopharyngeal swab • Bone marrow • Buffy coat • Tissue • 24-hour urine • Catheterized urine • Heparin whole blood or plasma
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
a.m. Daily
Report Available
3-4 days
Reference Range
| CMV DNA, QN PCR | Not Detected (IU/mL) |
| CMV DNA, QN PCR | Not Detected (Log IU/mL) |
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid. This is a quantitative molecular test, with a linear range of 200-2,000,000 IU/mL.
Performing Laboratory
| Quest Diagnostics Nichols Institute-Chantilly VA |
| 14225 Newbrook Drive |
| Chantilly, VA 20151-2228 |
