Cytomegalovirus DNA, Quantitative Real-Time PCR (QNCMV)

Test Code

LAB2222

Quest Code

10600

CPT Codes
87497

Preferred Specimen

1 mL whole blood or plasma collected in an EDTA (lavender- top) or ACD (yellow-top) tube or 1 mL plasma collected in EDTA PPT (white-top) tube or 1 mL serum collected in a red top tube (no gel) or serum separator tube (SST) or 1 mL CSF or amniotic fluid collected in a sterile leak-proof container.

Minimum Volume

1 mL

Instructions

Do not freeze whole blood or samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer's instructions for collection tube handling.

Transport Container

Transport tube

Transport Temperature

Refrigerated; random urine should be frozen.

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 6 days
Frozen -20° C: 84 days
Frozen -70° C: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen whole blood • Bronchial, nasopharyngeal, or tracheal lavage/wash • Synovial, pericardial, peritoneal, pleural or vitreous fluid • Sputum • Eye • Lesion (vesicle) aspirate • Nasal/nasopharyngeal swab • Bone marrow • Buffy coat • Tissue • 24-hour urine • Catheterized urine • Heparin whole blood or plasma

Methodology
Real-Time Polymerase Chain Reaction

Setup Schedule

a.m. Daily

Report Available

3-4 days

Reference Range

CMV DNA, QN PCR	Not Detected (IU/mL)
CMV DNA, QN PCR	Not Detected (Log IU/mL)

Clinical Significance

In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients. This is a quantitative molecular test, with a linear range of 34.5-10,000,000 IU/mL (1.54-7.00 log IU/mL).

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

14225 Newbrook Drive

Chantilly, VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.