Antiphospholipid Antibody Panel (APH)

Test Code

Quest Code

CPT Codes
86146 (x3),86147 (x3),86148 (x3)

Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Phosphatidylserine Antibodies (IgG, IgA, IgM)
Cardiolipin Antibodies (IgA, IgG, IgM)

Preferred Specimen
5 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
3 mL

Other Acceptable Specimens

Plasma (preferred): Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).

Serum: Centrifuge red-top tube 15 minutes at approximately 1500 x g within 24 hours of collection. Transfer serum into a plastic specimen vial.

Transport Container
Transport tube

Transport Temperature

Room temperature

Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic

Immunoassay (IA)

Reference Range
See individual tests

Clinical Significance
The Antiphospholipid Antibody Syndrome (APS) is a clinical-pathologic correlation that includes a clinical event (e.g., thrombosis, pregnancy loss, thrombocytopenia) and persistent positive Antiphospholipid Antibodies (IgM or IgG ACA >40 MPL/GPL, IgM or IgG anti-B2GPI antibodies, or a Lupus Anticoagulant). The IgA isotype has been implicated in smaller studies, but has not yet been incorporated into the APS criteria. International consensus guidelines suggest waiting at least 12 weeks before retesting to confirm antibody persistence. Reference J Thromb Haemost 2006: 4; 295.

Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA
14225 Newbrook Drive
Chantilly , VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.