Antiphospholipid Syndrome Diagnostic Panel (APSDP)

Test Code

Quest Code

CPT Codes
86147 (x3), 86146 (x3), 85730, 85613

Cardiolipin Antibodies (IgA, IgG, IgM)
Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Lupus Anticoagulant Evaluation with Reflex (PTT-LA and dRVVT with Reflex Confirmations)

If the PTT-LA test is prolonged (>40 seconds), then Hexagonal Phase Confirm is performed at an additional charge (CPT code(s): 85598).
If the Hexagonal Phase Confirm is positive or weakly positive, then a Thrombin Clotting Time will be performed at an additional charge (CPT code(s): 85670).
If dRVVT screen is prolonged (>45 seconds), then dRVVT Confirmation will be performed at an additional charge (CPT code(s): 85597).
If dRVVT Confirm is positive, then a dRVVT 1:1 Mix will be performed at an additional charge (CPT code(s): 85613).

Preferred Specimen
3 mL frozen plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
2 mL

Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.

Transport Container
Transport tube

Transport Temperature

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Grossly lipemic • Received room temperature • Received refrigerated • Serum

Immunoassay (IA) • Photo-Optical Clot Detection

Reference Range
See individual tests

Clinical Significance
This assay may be useful in supporting or ruling out a diagnosis of antiphospholipid syndrome.

Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA
14225 Newbrook Drive
Chantilly , VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.