A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Influenza A and B RNA, Qualitative, Real-Time RT-PCR (FLUPCR)
Test CodeLAB8413
Quest Code
16086
CPT Codes
87502
Preferred Specimen
Throat or nasopharyngeal swab submitted in 1 mL VCM (green-cap), or equivalent (UTM) tube or phosphate buffered saline or 0.9% physiological saline or 0.85 mL bronchial or tracheal lavage/wash or sputum collected in a sterile, leak-proof container
Minimum Volume
0.6 mL
Instructions
Upper respiratory swab samples in VCM, M4 or UTM transport media, PBS or 0.9 saline: Use only sterile dacron or rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap.
Sputum or bronchial lavage: Collect in a sterile container with a leak-proof cap.
Transport Container
VCM, UTM equivalent or plastic, leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Nasopharyngeal or throat swab in PBS
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: Unacceptable
All other samples
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: Unacceptable
All other samples
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs • Cotton swabs with wooden shaft • Amies liquid or gel transport used for bacterial culture
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Monday-sunday
Reference Range
Influenza A RNA, QL PCR | Not detected |
Influenza B RNA, QL PCR | Not detected |
Clinical Significance
This test is used to determine the presence of Influenza A or B in a patient's specimen. Organisms may be detected by PCR prior to diagnosis by immunological methods. PCR provides more rapid results than other methods, including culture.
Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA |
14225 Newbrook Drive |
Chantilly, VA 20151-2228 |