ADAMTS13 Activity with Reflex to Inhibitor (A13ARI)

Message
For inpatient tests, use test code ADA13B (LAB8554). This test for outpatients only.

Test Code
LAB8061

Quest Code
14532

CPT Codes
85397

Includes
If ADAMTS13 Activity is ≤30, then ADAMTS13 Inhibitor will be performed at an additional charge (CPT code(s): 85335).

Preferred Specimen
1 mL frozen plasma collected in a 3.2% sodium citrate (light blue-top) tube

Minimum Volume
0.5 mL

Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.

Transport Container
Transport tube

Transport Temperature
Frozen

Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen -20° C: 21 days
Frozen -70° C: 6 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature • Received refrigerated • Received thawed • EDTA plasma

Methodology
Immunoassay (IA)

FDA Status
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means.

Setup Schedule
Tuesday, thursday

Reference Range
ADAMTS13 Activity 68-163 % Activity
ADAMTS13 Inhibitor <0.4 BEU


Clinical Significance
ADAMTS-13 is a zinc metalloprotease that cleaves ultra large vWF multimers. Studies have shown that low levels of ADAMTS-13 activity are associated with thrombotic thrombocytopenic purpura (TTP), a life-threatening hematological condition characterized by a low platelet count, microvascular thrombi, red cell fragmentation, and renal complications. Congenital TTP is a rare inherited disease caused by mutations within the ADAMTS-13 gene, which result in the production of non-functional protein. The acquired form of TTP is an autoimmune-like disorder caused by the development of autoantibodies to ADAMTS-13 that inhibits enzyme activity.

Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA
14225 Newbrook Drive
Chantilly, VA 20151-2228




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.