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Progesterone, Immunoassay (PGST)
Test CodeLAB529
Quest Code
745
Alias/See Also
PGST
CPT Codes
84144
Preferred Specimen
1 mL serum in gold top tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plain red top tube
Instructions
DHEA supplementation causes a false elevation of values in this standard Immunoassay method for progesterone. Physicians using progesterone levels as a criterion for triggering ovulation in an IVF cycle in patients supplemented with DHEA should assess progesterone levels using a LC/MS/MS progesterone assay, such as order code 17183 performed at Quest Diagnostics, San Juan Capistrano.
Note: Do not add on progesterone testing to a barrier gel specimen older than 72 hours.
Note: Do not add on progesterone testing to a barrier gel specimen older than 72 hours.
Transport Container
Serum separator tube (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: 28 days
Do not add on Progesterone testing to a barrier gel specimen older than 72 hours
Refrigerated: 7 days
Frozen: 28 days
Do not add on Progesterone testing to a barrier gel specimen older than 72 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum stored on barrier gel for more than 24 hours ; Do not order progesterone testing as an add on to a barrier gel specimen older than 24 hours. Progesterone binds to barrier gel causing decreased values.
Methodology
Immunoassay
Setup Schedule
Monday through friday 4: 00 am to 5: 30 pm saturday 4: 00 am to 10: 00 am Monday through saturday
Report Available
3 days
Limitations
Heterophil antibodies may produce falsely-elevated results.
Reference Range
Male
<1.4 ng/mL
Female
Follicular Phase <1.0 ng/mL
Luteal Phase 2.6-21.5 ng/mL
Mid-Luteal Phase 4.4-28.0 ng/mL
Postmenopausal <0.5 ng/mL
Pregnancy
First Trimester 4.1-34.0 ng/mL
Second Trimester 24.0-76.0 ng/mL
Third Trimester 52.0-302.0 ng/mL
Children (<18 Years) Not established
Progesterone reference ranges established on post-pubertal patient population. Reference range not established for pre-pubertal patients using this assay. For pre-pubertal patients, the Nichols Progesterone, LC/MS/MS assay is recommended (Order code 17183).
<1.4 ng/mL
Female
Follicular Phase <1.0 ng/mL
Luteal Phase 2.6-21.5 ng/mL
Mid-Luteal Phase 4.4-28.0 ng/mL
Postmenopausal <0.5 ng/mL
Pregnancy
First Trimester 4.1-34.0 ng/mL
Second Trimester 24.0-76.0 ng/mL
Third Trimester 52.0-302.0 ng/mL
Children (<18 Years) Not established
Progesterone reference ranges established on post-pubertal patient population. Reference range not established for pre-pubertal patients using this assay. For pre-pubertal patients, the Nichols Progesterone, LC/MS/MS assay is recommended (Order code 17183).
Clinical Significance
Levels increase sharply during the luteal phase of the menstrual cycle. The level increases from 9 to 32 weeks of pregnancy.
Performing Laboratory
| Quest Diagnostics - South Region |
