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Herpes Simplex Virus Culture, Rapid Method with Reflex to Typing (HSVCUL)
Test CodeLAB946
Quest Code
2649
Alias/See Also
HSVCUL
CPT Codes
87255
Includes
If culture is positive, then typing will be performed at an additional charge (CPT code(s): 87140 per typing)
Preferred Specimen
• Vesicle, lesion fluid, scrapings or swab from the base of the lesion in Quest Diagnostics supplied V-C-M medium (green-cap) tube or equivalent
• 2 mL of CSF in sterile tube or V-C-M medium (green-cap) tube or equivalent
• Urogenital and respiratory specimens such as swabs, secretions or washings, should be inoculated into V-C-M medium (green-cap) tube or equivalent or submitted in a sterile leak-proof container
• Conjunctival or corneal scrapings in V-C-M medium (green-cap) tube or equivalent
• Tissues in sterile leak-proof containers with enough V-C-M medium (green-cap) tube or equivalent added to prevent drying of the specimen
• 2 mL of CSF in sterile tube or V-C-M medium (green-cap) tube or equivalent
• Urogenital and respiratory specimens such as swabs, secretions or washings, should be inoculated into V-C-M medium (green-cap) tube or equivalent or submitted in a sterile leak-proof container
• Conjunctival or corneal scrapings in V-C-M medium (green-cap) tube or equivalent
• Tissues in sterile leak-proof containers with enough V-C-M medium (green-cap) tube or equivalent added to prevent drying of the specimen
Minimum Volume
1 swab • 1 mL fluid • 1 g or 2mm
Other Acceptable Specimens
Multiple site swabs from newborns (rectal, mouth, eye, nasal, skin)
Instructions
Use only plastic shaft rayon or Dacron ® swabs. Specimens should be collected as soon as possible after clinical onset of disease. Highest viral titers are present during the acute illness. For V-C-M Medium Vials: 1. Aseptically remove cap from vial. 2. Aseptically place sample into the vial with medium. 3. Replace cap on vial and close tightly. 4. Label with appropriate patient information. 5. Send to the laboratory for immediate analysis.
Transport Container
Swabs: V-C-M medium (green-cap) tube or equivalent
CSF, respiratory fluids: Sterile, leak-proof container or V-C-M medium (green-cap) tube or equivalent
CSF, respiratory fluids: Sterile, leak-proof container or V-C-M medium (green-cap) tube or equivalent
Transport Temperature
Refrigerated
Specimen Stability
V-C-M medium (green-cap) tube or equivalent:
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Unpreserved, raw specimens:
Refrigerated: 72 hours
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Unpreserved, raw specimens:
Refrigerated: 72 hours
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Raw stool, dry swabs, gel-based transport systems, tissues or biopsies in formalin or other fixatives, DNA probe transport systems, Calcium alginate swabs, wooden shaft swabs, non gel-based bacterial transports, specimens received at room temperature, specimens received frozen at -20° C, sputum
Methodology
Spin Amplification Culture with Elvis ® Detection • Monoclonal Fluorescent Antibody Typing
Setup Schedule
7: 00 AM Monday, tuesday, wednesday, thursday, friday saturday, sunday
Report Available
9 days
Clinical Significance
Herpes Simplex Virus (HSV) is responsible for several clinically significant human viral diseases, with severity ranging from mild to fatal. Herpes Simplex Virus Type 1 infection are usually found above the waist. Herpes Simplex Virus Type 2 infections are more commonly seen in association with the genitalia, and surrounding areas, and are usually sexually transmitted. It is important to note however, that both Herpes Simplex types 1 and 2 have been involved in all disease manifestations and locations of the body, following introduction of the virus through broken skin or mucous membranes.
Performing Laboratory
| Quest Diagnostics - South Region |
