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Latex Allergy (k82), IMCAP (LATE)
Test CodeLAB84422
Quest Code
8927
CPT Codes
86003
Preferred Specimen
0.3 mL serum in gold top tube
Minimum Volume
0.15 mL/allergen
Other Acceptable Specimens
Plain red top tube
Transport Container
Serum separator tube (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Immunoassay (IA)
Setup Schedule
Cut-off: 8am
Report Available
3 days
Reference Range
See Medical Report
Clinical Significance
This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patients serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured. The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.
Performing Laboratory
Quest Diagnostics - South Region |