Culture, Mycobacteria with Fluorochrome Smear (AFBCUQ)

Test Code

85974

Quest Code

4554

CPT Codes
87116, 87206

Includes

Mycobacteria Culture, Concentration, Acid-Fast Bacilli Stain

An additional charge for Concentration (CPT code(s): 87015) will be added as appropriate.

Identification will be performed at an additional charge for each organism
CPT code(s) 87118: Matrix Assisted Laser Desorption/Ionization-Time of Flight or
CPT code(s) 87153: AFB Sequencing

If TB is isolated for the first time or after three months after the first isolate from a patient, susceptibilities will be performed at an additional charge (CPT code(s): 87190 per drug.

Preferred Specimen

10 mL body fluids or lower respiratory tract specimens or
10 mL gastric lavage or
2 mL CSF or
2 grams fresh (unfixed) tissue or
40 mL urine collected in a sterile leak-proof container or
1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile leak-proof container or
1 gram or 1 mL stool collected in a sterile leak-proof container

See the alphabetical listing in the Microbiology section of Specimen Collection & Transport Guide for further instructions.

Minimum Volume

2 mL body fluids or lower respiratory tract specimens • 2 mL gastric lavage • 1 mL CSF • 1 gram fresh (unfixed) tissue • 20 mL urine collected in a sterile leak-proof container • 1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile leak-proof container • 1 gram or 1 mL stool

Other Acceptable Specimens

Swabs in Amies liquid, Amies gel swab, Stuarts or ESwab transport systems, ONLY if tissue biopsy or wound aspirate is not available • Samples in anaerobic transport medium, ONLY if it is an irreplaceable sample type • Throat/oral/sputum swabs from cystic-fibrosis patients

Instructions

Tissue and biopsy material: Sterile leak-proof container with small amount of saline, no fixative or preservative.
Gastric lavage fluid: Sterile leak-proof container. Must be neutralized with sodium bicarbonate within 4 hours of collection.
Expectorated sputum: Instruct patient to gargle with water and cough deeply. Three separate early morning collections on at least three consecutive days are recommended.

Transport Container

Refer to Preferred Specimen(s) and Collection Instructions

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

24 hour pooled urine or sputum • Dry swabs • Specimens received in alcohol, formalin, EDTA, lithium heparin or conventional blood culture bottles • Throat/oral/sputum swabs from non-cystic-fibrosis patients • Urine specimens in preservative • Specimens received in expired transport • Specimens that leaked during transit • Specimens broken in transit

Methodology
Continuous Monitoring Broth Culture System and Conventional Culture • Microscopic Exam with Fluorochrome Stain

Setup Schedule

Mon-sat

Report Available

8 weeks

Reference Range

See Laboratory Report

Clinical Significance

There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy.

Performing Laboratory

Quest Diagnostics - South Region

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.