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Body Fluid Culture
Test CodePreferred Specimen
Minimum Volume
Instructions
Sterile Fluid
1. Clean needle puncture site with alcohol and disinfect with an iodine solution (1-2% tincture of iodine or a 10% solution of povidine-iodine 1% free iodine) to prevent introduction of infection. If tincture of iodine is used, remove with alcohol following procedure to avoid burn.
2. Physician will aseptically perform percutaneous aspiration to obtain pleural, pericardial, peritoneal, or synovial fluid.
3. Expel any air bubbles from syringe, and immediately inject specimen into either a transport system or remove needle from syringe.
4. Label container/syringe with patient’s name (first and last), medical record number (if available), date and time of collection, and type of specimen.
5. Maintain sterility and forward promptly.
Note: Specimen source is required on request form for processing.
Transport Container
Transport Temperature
Avoid extremes in temperature. If delays are unavoidable, hold the specimen at ambient temperature until processing. Do not refrigerate anaerobic cultures.
Do not transport syringe with needles attached. Specimen may be transferred to a sterile cup and processed STAT or material may be placed onto a swab and inserted into an anaerobic transport device.
If there is a delay in transport of 15 minutes, small specimen should be preserved in an anaerobic transport device.
Reference Range
Anaerobic bacterial culture: no growth
Performing Laboratory
Indiana Regional Medical Center