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Direct Antiglobulin Test
MessageDetects in vivo red blood cell sensitization with IgG Antibodies, complement, or both. If the test is positive it reflexes to two different test IgG monospecific and complement. If the IgG monospecific is positive an Acid Elution is performed to determine if the antibody can be identified. Only red cell antibodies can be identified with Blood Bank testing.
Test Code
DAT2
Includes
Polyspecifc IgG and C3d detects. If positive reflexes to two separate test for monospecific IgG detection and C3b, C3d detection. If the IgG monospecific is positive an Acid Elution is performed to determine if the antibody can be identified. Only red cell antibodies can be identified with Blood Bank testing.
Preferred Specimen
1ml
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Mislabelled
Clotted
Hemolyzed
Lipemic
Icteric
Clotted
Hemolyzed
Lipemic
Icteric
Methodology
Manually performed
Clinical Significance
The direct antiglobulin test (DAT) is used to detect in vivo red blood cell sensitization with immunoglobulin (IgG), complement, or both. The DAT is useful in the investigation of hemolytic transfusion reactions, hemolytic disease of the fetus and newborn, autoimmune hemolytic anemia, and drug-induced immune hemolytic anemia
Performing Laboratory
EPCH Blood Bank
