Leukocyte Adhesion Deficiency (LAD) Evaluation (CD15s,CD18), Flow Cytometry

Test Code

19815

Preferred Specimen

!!!-LOOK ALIKE-SOUND ALIKE-!!!
SODIUM HEPARIN, Green Top
Tube will read "Sodium Heparin"

Minimum Volume

2 mL

Instructions

Collect 5 mL of whole blood in a sodium heparin (green-top) tube. Mix thoroughly. Transport at room temperature immediately.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lipemia • Icterus • Clotted specimens • Received refrigerated • Received frozen specimens • Coagulants other than sodium heparin

Methodology
Flow Cytometry (FC)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Evening Sets up 7 days a week.

Reference Range

CD18 Expression (LAD-1)	≥90 %CD18
CD15s Expression (LAD-2)	≥90 %CD15s

Clinical Significance

LAD is an autosomal recessive immunodeficiency disorder associated with recurrent bacterial and fungal infections. Two forms of LAD have been described. LAD-1 is associated with defective expression of CD18 by neutrophils, whereas LAD-2 is associated with defective expression of CD15s (sialyl-Lewis X).

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.