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Post-Transplant, BK Virus DNA, Real-Time PCR, Urine
Test Code18052
Preferred Specimen
4.3 mL Urine in COBAS Media Tube
Minimum Volume
4.3 mL
Instructions
For Shipper collection please see detailed instructions in Test Resources. Neat urine must be immediately stabilized by transferring to a cobas® PCR urine sample tube (Liquid level must be between the black fill lines on the cobas® PCR Media tube label window).
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 90 days
Refrigerated: 90 days
Frozen: Unacceptable
Refrigerated: 90 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Under or over-filled cobas® PCR Media tubes • Neat urine
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Daily; Report available: 1 day
Reference Range
| BK Virus DNA, QN PCR | Not Detected (IU/mL) |
| BK Virus DNA, QN PCR | Not Detected (Log IU/mL) |
Clinical Significance
This test detects and quantitates BK Virus DNA in urine specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in urine (and plasma) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 200-100,000,000 IU/mL (2.30-8.00 log IU/mL).
