17-Hydroxyprogesterone Response to ACTH (Baseline, 30 min, 60 min)

Test Code

90492

Preferred Specimen

THREE Red Top Tubes

Minimum Volume

0.5 mL in THREE Red Top Tubes

Other Acceptable Specimens

Plasma collected in: EDTA (lavender-top), EDTA (royal blue-top), sodium heparin (green-top), or lithium heparin (green-top) tubes

Instructions

Draw baseline specimen. Draw another specimen 60 minutes after stimulation.

Serum separator tubes (SST) are unacceptable. Draw blood in a red-top tube (no gel). Separate serum after clotting. Ship serum refrigerated or frozen. Do not submit glass tubes.

Transport Container

Transport tubes

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Serum separator tubes (SST)

Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range

See Laboratory Report

Clinical Significance

17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.