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Aldosterone, Response to ACTH (Baseline, 30 Minute, 60 Minute)
Test Code90493
Preferred Specimen
Red Top
Minimum Volume
0.25 mL (x3)
Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top), sodium heparin (green-top), or lithium heparin (green-top) tubes
Instructions
Draw baseline specimen. Draw another specimen 30 minutes and 60 minutes after stimulation.
Serum separator tubes (SST) are unacceptable. Draw blood in a red-top tube (no gel).
Separate serum after clotting. Ship serum refrigerated or frozen. Do not submit glass tubes. Draw "upright" samples at least 1/2 hour after patient sits up.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory. This test cannot be collected at a Patient Service Center.
Serum separator tubes (SST) are unacceptable. Draw blood in a red-top tube (no gel).
Separate serum after clotting. Ship serum refrigerated or frozen. Do not submit glass tubes. Draw "upright" samples at least 1/2 hour after patient sits up.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory. This test cannot be collected at a Patient Service Center.
Transport Container
Transport tube (x3)
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Moderate to gross hemolysis • Serum separator tubes (SST)
Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Reference Range
See Laboratory Report
Clinical Significance
Approximately 1-2% of individuals with primary hypertension have primary hyperaldosteronism characterized by hypokalemia (low potassium) and low direct renin. Because serum aldosterone concentrations vary due to dietary sodium intake and body position, some physicians prefer measurement of 24-hour urine concentrations for aldosterone.
