von Willebrand Disease Profile

Test Code

135583

CPT Codes
85240, 85245, 85246, 85247

Includes

von Willibrand Factor Antigen
Ristocetin Cofactor
von Willebrand Factor Multimeric Analysis*
Factor VIII Activity, Clotting

Preferred Specimen

1 mL platelet-poor plasma collected in each of four separate 3.2% sodium citrate (light blue-top) tubes

Minimum Volume

1 mL (x4)

Instructions

Collect blood before coagulation factor replacement therapy is begun. Transport 4 separate frozen vials in the same shipping container.

Please submit a separate, frozen vial for each special coagulation assay ordered.

Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.

Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies

Refer to Quest Diagnostics Nichols Institute Directory of Services under Specimen Collection section, Coagulation Testing, for further information on specimen processing.

Transport Container

Plastic vials (x4)

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days

Methodology
Aggregation • Clotting Assay • Electrophoresis • Immunoturbidimetric

FDA Status
*This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Mon-Fri; Report available: 5 days

Reference Range

See individual tests

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.