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Bilirubin, Fractionated
Test Code7286
CPT Codes
82247, 82248
Includes
Bilirubin, Total
Bilirubin, Direct
Bilirubin, Indirect (calculated)
Bilirubin, Direct
Bilirubin, Indirect (calculated)
Preferred Specimen
1 mL serum collected in a serum separator tube (SST) - protect from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: Sodium heparin (green-top) tube or lithium heparin (green-top) tube - protect from light • Serum transferred to an amber or non-light protected transport vial
Instructions
Protect from light: Please place in an amber vial or wrap plastic vial in foil; avoid hemolysis.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: 90 days
Refrigerated: 72 hours
Frozen: 90 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
Set up: Daily; Report available: Next day
Reference Range
| Bilirubin, Total | |
| ≤1 Day | ≤5.1 mg/dL |
| 2 Days | ≤7.2 mg/dL |
| 3-5 Days | ≤10.3 mg/dL |
| 6-7 Days | ≤8.4 mg/dL |
| 8-9 Days | ≤6.5 mg/dL |
| 10-11 Days | ≤4.6 mg/dL |
| 12-13 Days | ≤2.7 mg/dL |
| 14 Days - 9 Years | 0.2-0.8 mg/dL |
| 10-19 Years | 0.2-1.1 mg/dL |
| ≥20 Years | 0.2-1.2 mg/dL |
| Bilirubin, Direct | ≤0.2 mg/dL |
| Bilirubin, Indirect | |
| ≤1 Day | ≤5.1 mg/dL (calc) |
| 2 Days | ≤7.2 mg/dL (calc) |
| 3-5 Days | ≤10.3 mg/dL (calc) |
| 6-7 Days | ≤8.4 mg/dL (calc) |
| 8-9 Days | ≤6.5 mg/dL (calc) |
| 10-11 Days | ≤4.6 mg/dL (calc) |
| 12-13 Days | ≤2.7 mg/dL (calc) |
| 14 Days - 9 Years | 0.2-0.8 mg/dL (calc) |
| 10-19 Years | 0.2-1.1 mg/dL (calc) |
| ≥20 Years | 0.2-1.2 mg/dL (calc) |
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstructive disease.
