BiliSeq

Instructions for storage of collection vials prior to specimen collection:

Preservative solution is light sensitive, always store vials in a dark place and at room temperature (+15° to +25° C) until expiration date.

Note: If any crystallization forms inside the collection vial or if the preservative solution turns yellow, appropriately discard the tube and collect the specimen using another collection tube.

Instructions for collecting a bile duct brushing sample for BiliSeq testing:

1. Take one vial containing 600 uL of stabilization fluid and clearly mark the vial with a typed label containing at least two patient identifiers.

2. Place the biopsy specimen within the collection vial and ensure the specimen is immersed in stabilization fluid. If submitting a brushing specimen, cut and place the end of the brush in the collection vial. Do not expose or rinse the specimen in Cytolyt® or another preservative.

3. After collection, secure the tube cap and invert several times to mix the collected sample.

4. Store the collected sample as follows:

• No longer than 3 hours at room temperature (+15° to +25° C)

• No longer than 24 hours at +2° to +8° C, i.e. in a refrigerator

• Up to 12 months at -15° to -25° C, i.e. in a freezer

Instructions for shipping a collected bile duct brushing sample for BiliSeq testing:

1. Ensure that all collection vials are labeled with at least two unique patient identifiers.

2. Ship with ice packs by overnight delivery. Shipping with dry ice is recommended for multiple tubes and when a delay in shipment is possible.

3. Include the completed test requisition form and the cytology report.