Busulfan IV Q12

Authorization letter needs to arrive before submission from requester.

- Draw minimum 1 mL (Pediatric) to 4 mL (Adult) of blood into 4-mL Sodium Heparin green top blood tubes.

- Busulfan degrades quickly at room temperature. Each sample should be kept in wet ice slurry or refrigerated at all times. Centrifuge them as soon as possible at 4 °C. Separate the plasma from each sample and transfer it to individual 4 mL plastic tubes. Immediately freeze these plasma samples at -20 °C.

- All samples have to be labeled with patient name, Medical Record #, date and actual clock time of the blood draw. Two unique identifiers and clock times are REQUIRED or samples can be rejected.

- Samples must be shipped on a minimum of 3 kg of dry ice using an overnight carrier. Samples that arrived thawed will not be analyzed.

- The REQUISITION SHEET for particular regimen, IV Q6 hr or test dose, IV Q24 hr, and Oral Q6 hr is provided below. It is required to be sent with the samples. Patient identifiers must be matched with those on the collected samples mentioned above. Also, our requisition serves as physician's order and must be signed by the attending physician or designee/caregiver according to the College American Pathologists (CAP) requirement.

The samples must arrive in the lab in the form of plasma, not whole blood.

The labels on the plasma tubes must consist of at least two patient identifiers, usually patient names and medical record numbers.

The samples must be shipped with a requisition which consists of the information of patient's demographics, dosing and regimen, sample collection clock times, and possible drug interactions.

The requisition must be signed by a physician or a designee as patient's care giver.