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Micronutrients, B-Vitamin Panel
Test Code10195
CPT Codes
82746, 84425, 82607, 84252, 84591 (x2), 84207
Includes
10188 - Micronutrient, Folate
10189 - Micronutrient, Vitamin B1 (Thiamine)
10190 - Micronutrient, Vitamin B2
10191 - Micronutrient, Vitamin B3
10192 - Micronutrient, Vitamin B5
10193 - Micronutrient, Vitamin B6
10194 - Micronutrient, Vitamin B12
10189 - Micronutrient, Vitamin B1 (Thiamine)
10190 - Micronutrient, Vitamin B2
10191 - Micronutrient, Vitamin B3
10192 - Micronutrient, Vitamin B5
10193 - Micronutrient, Vitamin B6
10194 - Micronutrient, Vitamin B12
Preferred Specimen
4 mL serum collected in a red-top tube (no gel) - protected from light and
3 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light and
2 mL frozen whole blood collected in an EDTA (lavender-top) tube - protected from light
3 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light and
2 mL frozen whole blood collected in an EDTA (lavender-top) tube - protected from light
Patient Preparation
Overnight fasting is required.
Avoid vitamin supplements for 24 hours prior to collection.
Minimum Volume
2 mL serum • 1 mL EDTA plasma • 1 mL EDTA whole blood
Instructions
Patients should be 18 years of age or greater.
Folate & Vitamin B12: Transfer 2 mL (1 mL min) serum into plastic transport tube, ship frozen.
Vitamin B2, B3, B5: Transfer 2 mL (1 mL min) serum into an amber vial (or wrap with aluminum foil), ship frozen.
Vitamin B6: Transfer 3 mL (1 mL min) EDTA plasma into an amber vial (or wrap with aluminum foil), ship frozen.
Vitamin B1: Transfer 3 mL (1 mL min) EDTA whole blood into an amber vial (or wrap with aluminum foil), ship frozen.
Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Remove serum or plasma from cells within 4 hours of collection.
Folate & Vitamin B12: Transfer 2 mL (1 mL min) serum into plastic transport tube, ship frozen.
Vitamin B2, B3, B5: Transfer 2 mL (1 mL min) serum into an amber vial (or wrap with aluminum foil), ship frozen.
Vitamin B6: Transfer 3 mL (1 mL min) EDTA plasma into an amber vial (or wrap with aluminum foil), ship frozen.
Vitamin B1: Transfer 3 mL (1 mL min) EDTA whole blood into an amber vial (or wrap with aluminum foil), ship frozen.
Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Remove serum or plasma from cells within 4 hours of collection.
Transport Container
Amber polypropylene or polyethylene tubes or
Neutral color polypropylene or
Polyethylene tubes wrapped in aluminum foil to protect from light
Neutral color polypropylene or
Polyethylene tubes wrapped in aluminum foil to protect from light
Transport Temperature
Frozen
Specimen Stability
Folate and Vitamin B12 Serum
Room temperature: 36 hours
Refrigerated: 7 days
Frozen: 28 days
Vitamin B2, B3 and B5 Serum
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Vitamin B6 Plasma
Room temperature: 6 hours
Refrigerated: 12 hours
Frozen: 6 days
Vitamin B1 Whole Blood
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 35 days
Room temperature: 36 hours
Refrigerated: 7 days
Frozen: 28 days
Vitamin B2, B3 and B5 Serum
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Vitamin B6 Plasma
Room temperature: 6 hours
Refrigerated: 12 hours
Frozen: 6 days
Vitamin B1 Whole Blood
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 35 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Received room temperature • Received refrigerated • Not protected from light • Serum separator tube (SST) • Plasma collected in gel tube
Methodology
Immunoassay (IA) • Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Setup Schedule
Set up and Report available: See individual assays
Reference Range
≥18 years
Reference range not available for individuals <18 years for this micronutrient test.
| Folate | >5.4 ng/mL |
| Vitamin B1 (Thiamine) | 78-185 nmol/L |
| Vitamin B2 | 6.2-39.0 nmol/L |
| Vitamin B3 | ≤110 ng/mL |
| Vitamin B5 | <275 ng/mL |
| Vitamin B6 | 2.1-21.7 ng/mL |
| Vitamin B12 | 200-1100 ng/mL |
Clinical Significance
See individual assays
