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Direct Antiglobulin Test (Direct Coombs)
Test CodeDAT
CPT Codes
86880 x2
Includes
Tests for antibodies (IgG) or complement components (C3) bound to red cells.
Preferred Specimen
PINK K2 EDTA
Minimum Volume
2-3 mL
Transport Container
PINK K2 EDTA
Transport Temperature
Room Temperature
Specimen Stability
Room Temp: 24 Hours; Refrigerated: 3 Days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, insufficient quantity, incorrect sample type
Methodology
Vision Swift (IgG), Manual Gel (C3b, C3d)
Setup Schedule
Monday - Sunday
Reference Range
Negative
Clinical Significance
Detects antibodies or complement proteins bound to red blood cells in vivo, often used to investigate hemolytic anemia or transfusion reactions.
Performed By
RFGH Laboratory
Performing Laboratory
RFGH Laboratory

