Direct Antiglobulin Test (Direct Coombs)

Test Code
DAT


CPT Codes
86880 x2

Includes
Tests for antibodies (IgG) or complement components (C3) bound to red cells.


Preferred Specimen
PINK K2 EDTA


Minimum Volume
2-3 mL


Transport Container
PINK K2 EDTA


Transport Temperature
Room Temperature


Specimen Stability
Room Temp: 24 Hours; Refrigerated: 3 Days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis, insufficient quantity, incorrect sample type


Methodology
Vision Swift (IgG), Manual Gel (C3b, C3d)

Setup Schedule
Monday - Sunday


Reference Range
Negative


Clinical Significance
Detects antibodies or complement proteins bound to red blood cells in vivo, often used to investigate hemolytic anemia or transfusion reactions.


Performed By
RFGH Laboratory

Performing Laboratory
RFGH Laboratory



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.