Transfusion Reaction Workup

Blood bank investigation of a suspected transfusion reaction requires immediate cessation of the transfusion, a clerical check, and sending the post-transfusion sample, along with the component bag and tubing, to the lab. Mandatory testing includes a clerical check, visual inspection for hemolysis, direct antiglobulin test (DAT), and retyping of donor/recipient RBCs.

TXRXN

Retests pre-transfusion ABO/Rh type and antibody screen (clerical/compatibility check); Post-transfusion ABO/Rh type, antibody screen, DAT and unit crossmatch compatibility

2 PINK K2 EDTA: 1 Pre-transfusion sample, 1 Post-transfusion sample, and component bag (if available)

3-6 mL EACH (pre/post samples)

PINK K2 EDTA

Room Temperature

Room Temp: 24 Hours; Refrigerated: 3 Days

Gross hemolysis, insufficient quantity, incorrect sample type

Monday - Sunday

Negative Interpretation (confirmed by Pathologist)

Transfusion reactions are adverse events associated with the transfusion of whole blood or one of its components. They range in severity from minor to life-threatening and can occur during a transfusion, termed acute transfusion reactions, or days to weeks later, termed delayed transfusion reactions. Transfusion reactions may be difficult to diagnose as they can present with non-specific, often overlapping symptoms. The most common signs and symptoms include fever, chills, urticaria, and itching. Some symptoms may resolve with little or no treatment. However, respiratory distress, high fever, hypotension, and hemoglobinuria may indicate a more serious reaction. All cases of suspected reactions should prompt immediate discontinuation of the transfusion and notification of the blood bank and treating clinician.