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Progesterone
Message Do not order progesterone testing to a barrier gel specimen older than 72 hours. Progesterone binds to barrier gel causing decreased values.
Test Code
LAB529
CPT Codes
84144
Preferred Specimen
4.5 ml (1.5 ml minimum) SST (gold) or no additive (red)
Minimum Volume
1.0 ml (500 ul minimum) serum
Instructions
After centrifugation, if not in gel barrier tube, pour off serum into an aliquot tube.
Transport Temperature
Room Temp
Specimen Stability
Separated from cells: 8 hours room temp, 48 hours refrigerated
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Icteric: >5 mg/dL bilirubin, Hemolysis: >500 mg/dL hemoglobin, Lipemia: >450 mg/dL triglycerides
Methodology
Immunoenzymatic
Setup Schedule
Mon- Sun
Reference Range
Male <1.4 ng/mL
Female
Follicular Phase <1.0 ng/mL
Luteal Phase 2.6-21.5 ng/mL
Postmenopausal <0.5 ng/mL
Pregnancy
First Trimester 4.1-34.0 ng/mL
Second Trimester 24.0-76.0 ng/mL
Third Trimester 52.0-302.0 ng/mL
Female
Follicular Phase <1.0 ng/mL
Luteal Phase 2.6-21.5 ng/mL
Postmenopausal <0.5 ng/mL
Pregnancy
First Trimester 4.1-34.0 ng/mL
Second Trimester 24.0-76.0 ng/mL
Third Trimester 52.0-302.0 ng/mL
Clinical Significance
Ovulation, and the presence of a functioning corpus luteum, can be demonstrated with serial determinations of
serum progesterone. Luteal phase dysfunction may be diagnosed when ovulation has occurred and there is
inadequate luteinization and reduced progesterone secretion
serum progesterone. Luteal phase dysfunction may be diagnosed when ovulation has occurred and there is
inadequate luteinization and reduced progesterone secretion
Performing Laboratory
Piedmont Athens Regional
