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Luteinizing Hormone
Test CodeLAB87
Alias/See Also
LH
CPT Codes
83002
Preferred Specimen
4.5 ml (1.5 ml minimum) lithium heparin (green); alternate-SST (gold) or Red (no additive)
Minimum Volume
1 ml (500 ul minimum) plasma; alternate-serum
Other Acceptable Specimens
Gold SST
Instructions
After centrifugation, if not in gel barrier tube, pour off serum into an aliquot tube.
Transport Temperature
Room Temp.
Specimen Stability
Separated from cells: 8 hours room temp, 5 days refrigerated
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Icteric: >10 mg/dL bilirubin, Hemolysis: >500 mg/dL hemoglobin, Lipemia: >1800 mg/dL triglycerides
Methodology
Immunoenzymatic
Setup Schedule
Mon- Sun
Reference Range
Males: 1.24-8.62 mIU/mL. Females (mIU/mL): Mid follicular phase: 2.12-10.89, Mid-cycle peak: 19.18-103.03, Mid-Luteal phase: 1.2-12.86, Postmenopausal: 10.87-58.64
Clinical Significance
Concentrations of hLH and hFSH are commonly determined in investigations of menstrual cycle, fertility, and
pubertal developmental abnormalities, such as premature ovarian failure, menopause, ovulatory disorders and
pituitary failure. In the male, elevated hLH and hFSH with low concentrations of
gonadal steroids may indicate testicular failure or anorchia. In Klinefelter’s syndrome hLH may be elevated due to
Sertoli cell failure.
pubertal developmental abnormalities, such as premature ovarian failure, menopause, ovulatory disorders and
pituitary failure. In the male, elevated hLH and hFSH with low concentrations of
gonadal steroids may indicate testicular failure or anorchia. In Klinefelter’s syndrome hLH may be elevated due to
Sertoli cell failure.
Performing Laboratory
Piedmont Athens Regional