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Vitamin D 25 (OH) Total
Test CodeV25
CPT Codes
82306
Preferred Specimen
Serum Separator (Gold/Yellow) Tube
Minimum Volume
1 mL
Transport Temperature
Refrigerated
Specimen Stability
​Room Temperature: Unacceptable
Refrigerated: 7 days
Frozen: 7 days
Refrigerated: 7 days
Frozen: 7 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis, Icterus, Lipemia
Reference Range
30.0-100.0 ng/mL
Clinical Significance
Deficient: <20.0 ng/mL
Insufficient: 20.0-29.9 ng/mL
Sufficient: 30.0-100.0 ng/mL
Upper Safety Limit: >100.0 ng/mL
Insufficient: 20.0-29.9 ng/mL
Sufficient: 30.0-100.0 ng/mL
Upper Safety Limit: >100.0 ng/mL
Performing Laboratory
This test is performed at the following locations:
- MercyOne Dakota Dunes Medical Laboratory
Last Updated: May 22, 2025