| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Factor VIII Activity, Chromogenic
Test Code16049
CPT Codes
85240
Preferred Specimen
1 mL frozen plasma collected in 3.2% sodium citrate (light-blue top tube)
Minimum Volume
0.5 mL
Instructions
Platelet-poor plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: 8 hours
Refrigerated: 8 hours
Frozen: 21 days
Refrigerated: 8 hours
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received room temperature • Received refrigerated • Received thawed
Methodology
Chromogenic Assay
Setup Schedule
Mon, Wed
Report Available
4-8 days
Reference Range
50-160 % normal
Clinical Significance
Preferred test in patients with Lupus anticoagulant, on heparin or direct thrombin inhibitors, or on Factor VIII concentrates.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |
