BK Virus DNA, Quantitative, Real-Time PCR, Plasma

Test Code

BKV

Quest Code

11274

CPT Codes
87799

Preferred Specimen

2 mL (1 mL minimum) plasma collected in an EDTA (lavender-top) tube
No other acceptable specimen types

Minimum Volume

1 mL

Other Acceptable Specimens

None

Instructions

Do not freeze whole blood or samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer's instructions for collection tube handling.

Transport Container

Transport tube

Transport Temperature

Plasma: Send Frozen

Specimen Stability

Room Temp Plasma: Unacceptable
Refrigerated Plasma: 6 days
Frozen Plasma: 60 months

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Sodium heparin (green-top) tube; lithium heparin (green-top) tube; unspun PPT tubes; frozen whole blood

Methodology
Real-Time Polymerase Chain Reaction (PCR)

Setup Schedule

Daily

Report Available

2-4 days

Reference Range

BK Virus DNA, QN PCR	Not Detected (IU/mL)
BK Virus DNA, QN PCR	Not Detected (Log IU/mL)

Clinical Significance

This test detects and quantitates BK Virus DNA in plasma specimens of kidney transplant recipients who are at risk of developing BKV-associated nephropathy (BKVAN) and associated hemorrhagic cystitis leading to kidney failure. Prospective viral load monitoring of BKV DNA in plasma (and urine) is useful to exclude a diagnosis of BKVAN and to monitor the course of BKV infection. The assay is calibrated to the First World Health Organization International Standard for BKV DNA with a Reportable Range of 21.5-100,000,000 IU/mL (1.33-8.00 log IU/mL).

Performing Laboratory

med fusion

2501 South State Hwy 121, Suite 1100

Lewisville, TX 75067-8188

Last Updated: November 27, 2024
Last Review: J. Jacobson, November 27, 2024

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.