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HIV-1/2 Ab Differentiation (Supplemental Use Only) with Reflex HIV 1 RNA, QL RT PCR
Test Code93170
Alias/See Also
Testing reflexes from the in-house HIV 1/2 Antibodies.
LAB00367
LAB00367
CPT Codes
86701, 86702
Includes
If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 RNA, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87535).
Preferred Specimen
3 mL serum
Minimum Volume
2 mL
Other Acceptable Specimens
Serum collected in: Red-top tube (no gel) • Plasma collected in: EDTA (lavender-top) tube
Instructions
Serum: Collect in a serum separator tube or red-top tube (no gel). The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic, screw-cap vial. Red-top tube (no gel) serum should be transferred to a plastic, screw-cap vial and submitted for testing.
Plasma: Collect in EDTA (lavender top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic, screw-cap vial, label as plasma, and submit for testing.
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Plasma: Collect in EDTA (lavender top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic, screw-cap vial, label as plasma, and submit for testing.
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Transport Container
Serum separator tube (SST)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 72 hours
Refrigerated: 5 days
Frozen: 30 days
Refrigerated: 5 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Frozen serum separator tube (SST)
Methodology
Immunoassay (IA)
Setup Schedule
Sat-thu
Reference Range
HIV-1 Antibody | Negative |
HIV-2 Antibody | Negative |
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |