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Quest AD-Detect® Apolipoprotein E (ApoE) Isoform, Plasma
Message**Plasma MUST be transfered to a Polypropylene Tube for transport! Ship FROZEN.**
Test Code
12563
Alias/See Also
LAB01200
CPT Codes
82542
Preferred Specimen
1 mL plasma collected in a K2 EDTA (lavender-top) tube
Minimum Volume
0.5 mL
Instructions
Collect blood into a K2 EDTA Plasma tube and mix by inversion 10 times. Centrifuge specimen to separate plasma from cells within 1 hour of collection. Carefully transfer plasma into a polypropylene tube or one of the Quest issued polypropylene pour-off/ transfer tubes. Do not transfer to conical tube. If unable to centrifuge within 1 hour of collection, store tube on ice. Store frozen.
Note: Quest Diagnostics does not perform this test for individuals under the age of 18. Pre-test and post-test counseling is strongly recommended.
Note: Quest Diagnostics does not perform this test for individuals under the age of 18. Pre-test and post-test counseling is strongly recommended.
Transport Container
Polypropylene transport tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 32 days
Refrigerated: 14 days
Frozen: 32 days
Methodology
Liquid Chromatography/Mass Spectrometry (LC/MS)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Wed
Report Available
4-12 days
Reference Range
Not established
Clinical Significance
Apolipoprotein E (ApoE) is a well-defined genetic risk factor for late-onset Alzheimer's disease (AD). The human APOE gene has three polymorphic alleles, E2, E3 and E4 that result in six different genotypes: E2/E2, E2/E3, E3/E3, E2/ E4, E3/E4 and E4/E4. About half of AD patients carry the E4 allele (compared with 14% in the general population), with the majority being heterozygotes (E3/E4). The number of inherited E4 alleles is associated with both increased disease risk and decreased average age of onset compared with inheritance of the E2 or E3 alleles. The differences between the three ApoE isoforms are based on two amino acids that affect its structure and hence the interaction and binding of the protein with various lipids and beta-amyloid (AB). ApoE and AB can co-localize in the brain, and therefore their complementary roles in AD have been studied extensively. Circulating plasma and CSF ApoE levels were recently found to be potential biomarkers for AD.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |