Blastomyces Antibody, Complement Fixation, Serum

Test Code
933


CPT Codes
86612

Preferred Specimen
1 mL serum


Minimum Volume
0.1 mL


Instructions
Note: For CSF use test code 5294 - Blastomyces Antibody Compliment Fixation, CSF.


Transport Container
Transport tube


Transport Temperature
Room temperature


Specimen Stability
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Grossly icteric


Methodology
Complement Fixation (CF)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Tues-sat


Report Available
4-7 days


Reference Range
Titer Interpretation
<1:8 Antibody Not Detected
≥1.8 Antibody Detected
Many proven cases of blastomycosis fail to react serologically; therefore, the absence of titer does not rule out the possibility of infection. It is recommended that acute and convalescent sera be submitted and assayed together to obtain a diagnostic interpretation of an acute infection, if clinically indicated.


Clinical Significance
Although titers of 1:8 and greater are considered positive, confirmed serological diagnosis of blastomycosis by the CF test requires a four-fold or greater increase in titer between acute and convalescent specimens, and lack of corresponding titers to C. immitis and H. capsulatum. Specificity can only be demonstrated by immunodiffusion procedures. A negative CF test does not rule out the diagnosis of active blastomycosis, as less than 50% of sera from patients with proven blastomycosis are positive by this assay.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.