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Cytomegalovirus DNA, Qualitative, Real-Time PCR
Test Code10601
Alias/See Also
LAB1332
CMV PCR QUALITATIVE
CMV PCR QUALITATIVE
CPT Codes
87496
Preferred Specimen
1 mL of any one of the following specimens:
Whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube or
Plasma collected in an EDTA (lavender-top), ACD B (yellow-top) or PPT EDTA (white-top) tube or
Serum or
CSF, bronchoalveolar lavage, eye fluids, amniotic fluid amniotic fluid supernatant, random urine collected in a sterile leak-proof container
Whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube or
Plasma collected in an EDTA (lavender-top), ACD B (yellow-top) or PPT EDTA (white-top) tube or
Serum or
CSF, bronchoalveolar lavage, eye fluids, amniotic fluid amniotic fluid supernatant, random urine collected in a sterile leak-proof container
Minimum Volume
0.5 mL
Other Acceptable Specimens
3 mm frozen fresh tissue
Instructions
Whole blood: Collect whole blood in sterile tubes containing EDTA or ACD as anticoagulant. Store refrigerated. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or Serum Separator Tube (SST®) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL, eye fluids: Collect in a sterile container and store refrigerated or frozen.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant or in plasma preparation tubes (PPT). Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap tubes and store refrigerated or frozen. If blood is collected in a PPT tube, centrifuge within 2 hours of collection and store refrigerated or frozen. It is not necessary to transfer the plasma from a PPT tube to aliquot tubes.
Serum: Collect in a red-top tube (no gel) or Serum Separator Tube (SST®) and store refrigerated or frozen.
CSF, amniotic fluid, urine, BAL, eye fluids: Collect in a sterile container and store refrigerated or frozen.
Transport Container
See Collection Instructions
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other sample types
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized specimens • Unspun PPT tube
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Daily
Reference Range
Not detected
Clinical Significance
In patients who are immunocompromised, CMV may cause disseminated, severe disease. CMV is also the most common cause of congenital viral infection in humans. Quantitative PCR methods may be useful in monitoring CMV replication in immunosuppressed patients or in determining the viral load of CMV in amniotic fluid.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |