Mycophenolic Acid, LC/MS/MS

Test Code

10662

CPT Codes
80180

Includes

Mycophenolic Acid and MPA Glucuronide

Preferred Specimen

1 mL serum collected in a red-top tube (no gel)

Patient Preparation
Optimum time to collect sample: 0.5 to 1 hour before next dose (trough) at steady-state (3-5 days after treatment with oral doses).

Minimum Volume

0.5 mL

Other Acceptable Specimens

Plasma collected in: Sodium heparin (green-top) tube or EDTA (lavender-top) tube

Instructions

Serum: Collect blood in a red-top tube (no gel). Allow blood to clot at 15-28° C for 20-30 minutes. Centrifuge at 25-28° C (2200-2500 rpm, 1000 x g) for 8-10 minutes. Transfer serum to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for more than 3 days are not acceptable.

Plasma: Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifugation at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for more than 3 days are not acceptable.

Transport Container

Transport tube

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 60 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Lipemia • Improperly labeled specimens • Specimens left at room temperature >72 hours • Gel barrier/serum separator tube (SST)

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

9: 00 a. M. Mon-sat

Report Available

2-3 days

Reference Range

Mycophenolic Acid	1.0-3.5 mcg/mL
MPA Glucuronide	35.0-100.0 mcg/mL

Clinical Significance

Mycophenolic acid is an immunosuppressant used in tissue transplants. It prevents graft rejection by the host's immune system. It is very important to monitor its level. Too little of this drug will cause graft rejectoin, while too much will lead to infection. Monitoring its level is essential to optimize therapeutic effects, avoid toxicity, and assure compliance.

Performing Laboratory

Quest Diagnostics Nichols Institute

27027 Tourney Road

Valencia, CA 91355-5386

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.