A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Bartonella DNA, Qualitative Real-Time PCR
Test Code11108
Alias/See Also
LAB1322
CPT Codes
87471
Includes
Bartonella henselae and Bartonella quintana
Preferred Specimen
0.7 mL whole blood collected in an EDTA (lavender-top) tube or 3x3 tissue biopsy collected in a plastic, sterile, leak-proof container
Minimum Volume
0.3 mL whole blood
Transport Container
Whole blood: EDTA (lavender-top) tube
Tissue: plastic, sterile, leak-proof container
Tissue: plastic, sterile, leak-proof container
Transport Temperature
Whole blood: Refrigerated (cold pack)
Tissue: Frozen
Tissue: Frozen
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparinized whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Mon-sat
Report Available
2-4 days
Limitations
B. quintana and B. henselae as well as chlamydia species and Coxiella burnetii may cross-react and produce false-positive results.
Reference Range
B. henselae DNA, QL PCR | Not detected |
B. quintana DNA, QL PCR | Not detected |
Clinical Significance
Bartonella DNA PCR is a highly specific and sensitive method to detect the presence of Bartonella species DNA in clinical specimens. This assay can differentiate between Bartonella henselae and Bartonella quintana. The diagnosis of Bartonella infection should not rely solely on the result of a PCR assay. A negative PCR result indicates only the absence of Bartonella species DNA in the sample tested and does not exclude the diagnosis of the disease. Patients with a positive PCR result should be evaluated with other tests to further establish the diagnosis of the disease. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests.
Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
33608 Ortega Highway |
San Juan Capistrano, CA 92675-2042 |