| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
BK Virus DNA, Quantitative, Real-Time PCR, Plasma
Test Code11274
Alias/See Also
LAB1374
CPT Codes
87799
Preferred Specimen
0.7 mL plasma collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube or
0.7 mL serum collected in a serum separator tube (SST) or
0.7 mL whole blood collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
0.7 mL serum collected in a serum separator tube (SST) or
0.7 mL whole blood collected in an EDTA (lavender-top) tube or ACD (yellow-top) tube
Minimum Volume
0.3 mL
Instructions
For CSF order test code 18901-BK Virus DNA, Quantitative Real-Time PCR, CSF and for urine order test code 16581-BK Virus DNA, Quantitative Real-Time PCR, Urine.
Plasma: Collect blood in sterile tubes containing EDTA as an anticoagulant, or in plasma preparation tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, transport aliquot tubes. Freeze and ship frozen.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, transport aliquot tubes. Freeze and ship frozen.
Whole blood: Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.
Plasma: Collect blood in sterile tubes containing EDTA as an anticoagulant, or in plasma preparation tube (PPT). Collect whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, transport aliquot tubes. Freeze and ship frozen.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum within 1 hour of collection. Transfer serum to sterile, transport aliquot tubes. Freeze and ship frozen.
Whole blood: Collect using standard procedures. Store samples refrigerated following collection. Do not freeze whole blood.
Transport Container
Tranport tube
Transport Temperature
Plasma or serum: Frozen
Whole blood: Refrigerated (cold packs)
Whole blood: Refrigerated (cold packs)
Specimen Stability
Plasma and serum
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sodium heparin (green-top) tube • Lithium heparin (green-top) tube
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Daily
Report Available
3 days
Reference Range
| BK Virus DNA,QN RT PCR | Not Detected (copies/mL) |
| BK Virus DNA,QN RT PCR | Not Detected (Log copies/mL) |
Clinical Significance
This test is used to determine the presence of BK Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, and not the host's reaction to the virus. Polyomavirus BK DNA detection in urine or plasma is associated with an increased risk of graft rejection in renal recipients. Quantitative testing may indicate change in risk over time. This is a quantitative molecular test, with a linear range of 500-500,000,000 copies/mL.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |