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Free Progesterone
Test Code15072
Alias/See Also
LAB00986
CPT Codes
84144 (x2)<br><strong>This test is not available for New York patient testing.</strong>
Includes
Free Progesterone
% Free Progesterone (% of Total Progesterone)
Progesterone, Total
% Free Progesterone (% of Total Progesterone)
Progesterone, Total
Preferred Specimen
3 mL serum
Patient Preparation
Patient should not be on any Corticosteroid, ACTH, Estrogen, or Gonadotropin medication, if possible, for at least 48 hours prior to collection of specimen.
Minimum Volume
2 mL
Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top) tube
Instructions
Specimen should be collected and separated as soon as possible
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: 1 hour
Refrigerated: 7 days
Frozen: 6 months
Refrigerated: 7 days
Frozen: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Grossly icteric
Methodology
Radioimmunoassay following ultrafiltration of specimens
FDA Status
This test was developed and its performance characteristics determined by Inter Science Institute. Values obtained with different methods, laboratories, or kits cannot be used interchangeably with the results on this report. The results cannot be interpreted as absolute evidence of the presence or absence of malignant disease.
This test is not available for New York patient testing.
Setup Schedule
Daily
Report Available
12 days
Reference Range
Free Progesterone
% Free Progesterone (% of Total Progesterone)
Progesterone, Total
| Male | 5-20 ng/dl |
| Female | Up to 20 ng/dl |
% Free Progesterone (% of Total Progesterone)
| Male | 11-57 % |
| Female | 5-64 % |
Progesterone, Total
| Male | 5-50 ng/dl |
| Female - Follicular | 10-100 ng/dl |
| Female - Luteal | 200-1800 ng/dl |
Clinical Significance
Progesterone is a Progestin produced primarily from enzymatic metabolism of Pregnenolone. It is enzymatically converted to 17-Hydroxy Progesterone and 11-Deoxycorticosterone. It is secreted by both the gonads and the adrenal glands. It is mostly bound to Cortisol Binding Globulin and Albumin, but a small percentage is present in the "Free" form. This "Free" Progesterone is the bioactive moiety. It is excreted into the urine primarily as "Free" unconjugated Progesterone and as Pregnanediol. Progesterone is responsible for cellular changes in the cervix, vagina, and uterus. Levels are lowest in the follicular phase and increase rapidly following the luteal surge. Increased Progesterone inhibits ovulation. "Free" Progesterone increases greatly during pregnancy reaching about 20% of the total Progesterone concentration at delivery. Measurement of Progesterone can be useful to monitor fertility, corpus luteum function, endometrial development, and be helpful in in-vitro fertilization patients.
References:
1. S Batra, LP Bengtsson, H Grundsell, and N-O Sjoberg. Levels of Free and Protein-Bound Progesterone in Plasma during Late Pregnancy. Journal of Clinical Endocrinology and Metabolism 42: 1041, 1976.
2. CJ Munro, GH Stabenfeldt, JR Cragun, LA Addiego, JW Overstreet, and BL Lasley. Relationship of Serum Estradiol and Progesterone Concentrations to the Excretion Profiles of Their Major Urinary Metabolites as Measured by Enzyme Immunoassay and Radioimmunoassay. Clinical Chemistry 37: 38-44, 1991.
References:
1. S Batra, LP Bengtsson, H Grundsell, and N-O Sjoberg. Levels of Free and Protein-Bound Progesterone in Plasma during Late Pregnancy. Journal of Clinical Endocrinology and Metabolism 42: 1041, 1976.
2. CJ Munro, GH Stabenfeldt, JR Cragun, LA Addiego, JW Overstreet, and BL Lasley. Relationship of Serum Estradiol and Progesterone Concentrations to the Excretion Profiles of Their Major Urinary Metabolites as Measured by Enzyme Immunoassay and Radioimmunoassay. Clinical Chemistry 37: 38-44, 1991.
Performing Laboratory
| Interscience Institute |
| 944 W Hyde Park Blvd |
| Inglewood, CA 90302-3308 |