HLA-B27 DNA Typing

Test Code

15584

Alias/See Also

LAB1274

CPT Codes
81374

Preferred Specimen

5 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

3 mL

Other Acceptable Specimens

Whole blood collected in: Sodium heparin (green-top) tube, EDTA (pink-top) tube, EDTA (royal blue-top) tube, or 3.2% sodium citrate (light blue-top) tube

Instructions

A separate EDTA (lavender-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA tube, please submit a separate tube for this test. Do not open tube.

Transport Container

EDTA (lavender-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen • Clotted

Methodology
Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes

Setup Schedule

Daily

Report Available

6 days

Reference Range

Negative

Clinical Significance

This test is used in the evaluation of genetic risk for Ankylosing Spondylitis, uveitis, and several other autoimmune disorders.

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

14225 Newbrook Drive

Chantilly, VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.