| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Desmoglein Antibodies (1 and 3)
Test Code16033
Alias/See Also
LAB00877
DESMO 1/3
DESMO 1/3
CPT Codes
83520 (x2)
Preferred Specimen
0.3 mL serum
Patient Preparation
Blood should be collected fasting or at least one hour after meals to avoid lipemic serum
Minimum Volume
0.1 mL
Instructions
Aseptically collect 5 to 8 mL of blood by approved venipuncture procedures.
Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Transport Container
Transport tube
Transport Temperature
Refrigerated (cold packs)
Note: Specimen stabilized upon receipt
Frozen acceptable, room temperature unacceptable
Note: Specimen stabilized upon receipt
Frozen acceptable, room temperature unacceptable
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia • Icterus • Microbial growth • Received room temperature
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
Setup Schedule
Wed, fri
Report Available
3-9 days
Reference Range
Desmoglein 1 Antibody
Desmoglein 3 Antibody
| Negative | <14 U/mL |
| Equivocal | 14-20 U/mL |
| Positive | >20 U/mL |
Desmoglein 3 Antibody
| Negative | <9 U/mL |
| Equivocal | 9-20 U/mL |
| Positive | >20 U/mL |
Clinical Significance
To detect the presence of auto antibody specific to Desmoglein 1 and/or 3 in a patients serum as an aid to diagnose type of pemphigus
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |