Adenovirus DNA, Qualitative, Real-Time PCR

Test Code

16046

Alias/See Also

LAB782

CPT Codes
87798

Preferred Specimen

Throat or nasopharyngeal swab submitted in 1 mL M4 media, VCM (green-cap), or equivalent (UTM) tube

Minimum Volume

3 cubic mm tissue • 0.6 mL all other specimen types

Other Acceptable Specimens

1 mL serum • 1 mL whole blood or plasma collected in EDTA (lavender-top) tube • 1 mL CSF, sputum, or urine collected in a sterile leak-proof container • 1 mL bronchial, nasopharyngeal, or tracheal lavage/wash collected in a sterile plastic leak-proof container • 3 cubic mm tissue collected in a sterile plastic leak-proof container • Eye swab (conjunctival) in an M4, VCM (green-cap) container or equivalent

Transport Container

M4, VCM (green-cap), or equivalent (UTM) tube

Transport Temperature

Tissue and sputum: Frozen
All other specimens: Refrigerated (cold pack)

Specimen Stability

Whole blood and urine
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

Sputum
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days

All other specimens
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Sodium heparin (green-top) tube • Lithium heparin (green-top) tube • Calcium alginate swabs

Methodology
Real-Time Polymerase Chain Reaction

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Daily

Report Available

3 days

Reference Range

Not detected

Clinical Significance

This test is designed to detect multiple strains of adenovirus, including adenovirus 40 and 41, but it does not differentiate among strains. Organisms may be detected by PCR prior to diagnosis by immunological methods. PCR provides more rapid results than other methods and is the preferred method for the detection of acute adenovirus infection.

Performing Laboratory

Quest Diagnostics Nichols Institute-San Juan Capistrano, CA

33608 Ortega Highway

San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.