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HLA Typing for Celiac Disease
Test Code17135
Alias/See Also
LAB822
CPT Codes
81382 (x2), 81376 (x2)<br><strong>CPT coding may differ dependent on payer rules which may impact prior authorization testing.</strong><br><strong>Please direct any questions regarding CPT coding to the payer being billed.</strong>
Includes
HLA-DQ2 (DQA1*05/DQB1*02)
HLA-DQ8 (DQA1*03/DQB1*0302)
HLA-DQA1*
HLA-DQB1*
HLA-DQ8 (DQA1*03/DQB1*0302)
HLA-DQA1*
HLA-DQB1*
Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL
Other Acceptable Specimens
Whole blood collected in: Sodium heparin (green-top) tube, EDTA (pink-top) tube, EDTA (royal blue-top) tube, or 3.2% sodium citrate (light blue-top) tube
Instructions
A separate EDTA (lavender-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA tube, please submit a separate tube for this test. Do not open tube.
Transport Container
EDTA (lavender-top) tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Clotted
Methodology
Polymerase Chain Reaction Amplification followed by Sequence Specific Oligonucleotide Probes (PCR-SSO)
Setup Schedule
Daily
Report Available
6 days
Reference Range
See Laboratory Report
Clinical Significance
Celiac disease is a multigenic immune-mediated enteropathy triggered by dietary proteins, commonly known as glutens, present in wheat, barley, and rye. Varied clinical manifestations begin either in childhood or adult life. Its prevalence in the united states ranges from 0.5 to 1%. Celiac disease is strongly associated with the HLA genetic region. Approximately 90% of celiac patients express the HLA-DQ2 molecule. Most of the DQ2 negative patients express the HLA-DQ8 molecule. Gluten peptides presented by these HLA molecules induce an abnormal mucosal immune response and tissue damage. The HLA-DQ molecules are heterodimers of an alpha and a beta chain. The beta chain of HLA-DQ2 is coded by HLA-DQB1*02 and of DQ8 by HLA-DQB1*0302. HLA-DQB1 genotyping allows clinicians to evaluate the genetic predisposition for celiac disease in a patient.
Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA |
14225 Newbrook Drive |
Chantilly, VA 20151-2228 |