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Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Message*Take to processing to be spun and frozen ASAP.
Test Code
30340
Alias/See Also
LAB1179
CPT Codes
86146 (x3)
Preferred Specimen
3 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
1.5 mL
Other Acceptable Specimens
Serum
Instructions
Plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 5 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic
Methodology
Immunoassay (IA)
Limitations
Factor VIII inhibitors may cause a false-positive lupus anticoagulation test. The temporary presence of antiphospholipid antibodies may accompany infections or certain drugs.
Reference Range
| B2-Glycoprotein I (IgG) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
| B2-Glycoprotein I (IgA) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
| B2-Glycoprotein I (IgM) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
Clinical Significance
Beta-2-Glycoprotein 1 (also known as apolipoprotein H), is a cofactor in antiphospholipid antibody binding and is the critical antigen in the antiphospholipid antibody syndrome. The presence of high titer IgG/IgM beta-2-glycoprotein 1 antibodies is one criteria for the diagnosis of antiphospholipid antibody syndrome.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |