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14-3-3 Protein, CSF (Prion Disease)
Message**FILLOUT FORM**
https://case.edu/medicine/pathology/sites/case.edu.pathology/files/2020-02/Test%20Request%20Form%20With%20Billing%20Info%20%282%29.pdf
https://case.edu/medicine/pathology/sites/case.edu.pathology/files/2020-02/Test%20Request%20Form%20With%20Billing%20Info%20%282%29.pdf
Test Code
37989
Alias/See Also
LAB8230
14-3-3 PROT CREUTZFELDT-JAKOB (CSF)
14-3-3 PROT CREUTZFELDT-JAKOB (CSF)
CPT Codes
0035U, 83520, 84394
Preferred Specimen
2 mL frozen CSF collected in a sterile screw-cap vial
Patient Preparation
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
Minimum Volume
1 mL
Other Acceptable Specimens
CSF collected Sarstedt CSF false-bottom tube
Instructions
The patient must be 12 years of age or older.
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: Indefinitely
Refrigerated: Unacceptable
Frozen: Indefinitely
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bloody and insufficient sample quantity • Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA
Methodology
Immunoassay (IA)
FDA Status
These tests were developed and their performance characteristics determined by the NPDPSC, and have not been cleared or approved by the FDA. These assays should be used in conjunction with other clinical, pathological and laboratory findings.
Setup Schedule
Tues, thurs
Report Available
21 days
Reference Range
| RT-QUIC (CSF) | Negative |
| T-TAU Protein (CSF) | 0-1149 pg/mL |
| 14-3-3 Protein (CSF) | 173-1999 AU/mL |
| Likelihood of prion disease | See Laboratory Report |
Clinical Significance
In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.
Performing Laboratory
Natl Prion Disease Path
W Reserve Univ 2085 Adelbert Rd
RM 418
Cleveland, OH 44106-4907