14-3-3 Protein, CSF (Prion Disease)


Test Code

Alias/See Also

CPT Codes
0035U, 83520 (x2)

Preferred Specimen
5 mL frozen CSF

Patient Preparation
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.

Minimum Volume
2 mL

Collect CSF by lumbar puncture. Discard first 2 mL that flows from tap. Collect next 5 mL CSF, avoiding bloody tap. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours). The NPDPSC requests a urine sample for research purposes with all CSF samples if available.

*** Ship Monday through Wednesday ***

Note: Patient is also asked to submit a frozen urine sample (100-200 mL) for validation of a recently published diagnostic test on Creutzfeldt-Jakob disease; no report will be issued. Please inform patient and/or family that urine is used for research on a diagnostic test and obtain oral consent.

Urine: ship double boxed using a styrofoam container with sufficient dry ice (5 lbs/24 hours).

Transport Container
Transport tube

Transport Temperature

Specimen Stability
Room temperature: 24 hours
Refrigerated: 14 days
Frozen: Indefinitely

Immunoassay (IA)

FDA Status
These tests were developed and their performance characteristics determined by the NPDPSC, and have not been cleared or approved by the FDA. These assays should be used in conjunction with other clinical, pathological and laboratory findings.

Setup Schedule
Tues, thurs

Report Available
21 days

Reference Range
RT-QUIC (CSF) Negative
T-TAU Protein (CSF) 0-1149 pg/mL
14-3-3 Protein (CSF) <30-1999 AU/mL

Clinical Significance
In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.

Performing Laboratory
Natl Prion Disease Path
W Reserve Univ 2085 Adelbert Rd
RM 418
Cleveland, OH 44106-4907

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.