A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Biotin (Vitamin B7)
Message*Protect from light.
Test Code
391
Alias/See Also
LAB00335
CPT Codes
84591
Preferred Specimen
2 mL frozen serum - protect from light
Minimum Volume
1 mL
Instructions
Allow sample to clot for 30 minutes, spin at 3000 rpm for 10 minutes and transfer serum to a plastic amber vial. If amber vial is not available, wrap tube in aluminum foil to protect from light. Freeze within 30 minutes and send frozen.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 14 days
Refrigerated: 7 days
Frozen: 14 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia • Received room temperature • Not protected from light • Samples other than serum
Methodology
Bioassay (BA)
FDA Status
The performance characteristics of the listed assay was validated by BioAgilytix Diagnostics. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval. BioAgilytix Diagnostics is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one.
Setup Schedule
Mon, thurs
Report Available
7 days
Reference Range
<12 years | 100.0-2460.2 pg/mL |
≥12 years | 221.0-3004.0 pg/mL |
Clinical Significance
Biotin, vitamin B7, or vitamin H, is a water soluble vitamin. The vitamin plays a role in the transferring of carbon dioxide in the metabolism of fat, carbohydrate and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone by the action of biotinyl-transferase. Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism. Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake [RDI of 101.9(c) (8) (IV)] for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis. Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention.
Performing Laboratory
Cambridge Biomedical Research Group |
1320 Soldiers Field Road |
Boston, MA 02135-1020 |