Factor VIII Inhibitor Panel

*Collect a total of 4 Lt blue top tubes for this test!!
*Take to processing ASAP

40083

LAB00174
 

Factor VIII Inhibitor, EIA Screen and Factor VIII Activity, Clotting

If Factor VIII Inhibitor, EIA Screen is positive, the Nijmegen Assay will be performed at an additional charge (CPT code(s): 85335).

2 mL plasma collected in 3.2% sodium citrate (Light blue- top) in each of two separate tubes

1 mL (x3)

Submit a total of 4 mL (2 mL in each of 2 tubes).
Centrifuge light blue-top tube 15 minutes at approx. 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcl). Freeze immediately and ship on dry ice.

Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies.

Plastic screw-cap vials (x3)

Frozen

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 14 days

Received room temperature • Received refrigerated

The presence of alloantibodies against factor VIII activity is a complication of treatment in hemophilia A, while the presence of autoantibodies may develop spontaneously in patients with acquired Factor VIII-C deficiency. The presence of Factor VIII inhibitor may lead to the neutralization (inactivation) of transfused or endogenous Factor VIII activity. The detection and magnitude of Factor VIII inhibitor is of great importance in the care of these patients. Factor VIII inhibitor EIA assay provides a quick method for the detection of Factor VIII inhibitor with less sample volume.