| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Platelet Antibody, Direct, Flow Cytometry
MessageROOM TEMP ONLY!
Do NOT refrigerate or freeze after collection.
if platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
Do NOT refrigerate or freeze after collection.
if platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
Test Code
5019
Alias/See Also
LAB1177
PLT ABD
PLATELET ANTIBODY
PLT ABD
PLATELET ANTIBODY
CPT Codes
86023
Preferred Specimen
7 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
5 mL
Instructions
Do not refrigerate or freeze.
Specimens submitted in ACD (yellow-top) tubes will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood.
Specimens submitted in ACD (yellow-top) tubes will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.
| Platelet Count | Minimum Volume |
| ≥45,000 | 2 mL |
| 20,000-45,000 | 5 mL |
| ≤20,000 | 10 mL |
Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood.
Transport Container
EDTA (lavender-top) tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 6 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • ACD (yellow-top) tubes
Methodology
Flow Cytometry (FC)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Daily
Report Available
3-4 days
Reference Range
| Anti-IgG | Negative |
Clinical Significance
Thrombocytopenia that is refractory to platelet transfusions may be due to direct platelet antibody. Testing is useful to differentiate immune from non-immune disorders.
Performing Laboratory
| Quest Diagnostics Nichols Institute-San Juan Capistrano, CA |
| 33608 Ortega Highway |
| San Juan Capistrano, CA 92675-2042 |