5' Nucleotidase

Test Code

671

Alias/See Also

LAB00475
5NT

CPT Codes
83915

Preferred Specimen

1 mL serum

Minimum Volume

0.2 mL

Instructions

Centrifuge and transfer serum specimens to a clean, plastic transport tube. Freeze and ship frozen.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 14 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received room temperature

Methodology
Colorimetric (C) • Kinetic

Setup Schedule

Sun-fri

Reference Range

0-10 U/L

Clinical Significance

5'-Nucleotidase (5'-NT) assay kit is for the determination of 5'-NT activity in human serum samples. 5'-NT is an enzyme catalyzing the hydrolysis of nucleoside-5'-mono- phosphates to nucleosides and inorganic phosphate. The enzyme is widely distributed in human and animal tissues. The activity present in sera is released from membranes of liver cells by bile salts and has been used as a marker for liver disease. Healthy subjects have a 5'-NT activity in the range of 0-10 U/L. Increased enzyme levels in sera are associated with certain forms of liver disease, such as intra- or extra-hepatic obstruction and particularly in cases of hepatic carcinoma and in mastectomy patients with recurrent metastases. The diagnostic value of 5'-NT has been shown to be superior to other liver enzymes, especially in cases of liver metastasis.

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

14225 Newbrook Drive

Chantilly, VA 20151-2228

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.