Nicotine and Cotinine, Urine

Test Code

Alias/See Also

CPT Codes
80323 (HCPCS: G0480)

Preferred Specimen
1 mL random urine

Minimum Volume
0.5 mL

Transport Container
Plastic urine container

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 30 days

Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
10: 00 a. M. Mon, wed, fri

Report Available
2-4 days

Reference Range
Nicotine, Urine
Smoker 200-700 ng/mL
Nonsmoker ≤17 ng/mL

Cotinine, Urine
Smoker 300-1300 ng/mL
Nonsmoker ≤20 ng/mL

Individuals exposed to second-hand or passive tobacco smoke may demonstrate concentrations of nicotine and cotinine greater than those indicated for nonsmokers.

Clinical Significance
This assay is used for the detection of nicotine and cotinine in urine to determine the tobacco exposure status of the individual. Nicotine has a short half-life of approximately forty minutes; its presence may indicate recent tobacco exposure. Cotinine, the major nicotine metabolite, has a half-life of 24 hours and is detectable for several days after cessation of tobacco exposure.

Performing Laboratory
Quest Diagnostics Nichols Institute
27027 Tourney Road
Valencia, CA 91355-5386

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.