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RED BLOOD CELL MEMBRANE EVALUATION
Messagehttps://www.mayomedicallaboratories.com/it-mmfiles/Hereditary_Spherocytosis_Patient_Sheet.pdf
(TEST CODE: RBCME)
Test Code
Alias/See Also
Mayo TC: RBCME
Band 3
EMA Binding
CPT Codes
85557,88184,85060
Includes
Spherocytosis Interpretation
Band 3 Fluorescence Staining, RBC
Peripheral Blood Smear Review
Shipping Control Vial
Preferred Specimen
Patient:
Specimen Type: Whole blood and 2 Wright-stained Slides
Container/Tube: 2 vials of 4 mL lavender-top tubes
Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
2. Label clearly on outermost label normal control.
Patient Preparation
THIS TEST MUST HAVE A PATIENT CONTROL DRAWN. SEE "NORMAL SHIPPING CONTROL" ABOVE
Minimum Volume
Instructions
Instructions
Patient:
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
2. Send specimen in original tube. Do not aliquot.
3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
4. Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.
Additional Information: Patient's age and sex are required.
Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
2. Label clearly on outermost label normal control.
3. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
4. Send specimen in original tube. Do not aliquot.
5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
Transport Container
Transport Temperature
Specimen Stability
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
No shipping control.
Methodology
Osmotic fragility and eosin-5-maleimide (EMA) binding (Band3) flow cytometry testing.
Report Available
Limitations
*Must draw a patient normal control!
Clinical Significance
Performing Laboratory
Mayo Clinical Laboratories