RED BLOOD CELL MEMBRANE EVALUATION

Message

HAVE PATIENT COMPLETE RED CELL MEMBRANE DISORDERS FORM FOUND ON MAYO WEBSITE
https://www.mayomedicallaboratories.com/it-mmfiles/Hereditary_Spherocytosis_Patient_Sheet.pdf
(TEST CODE: RBCME)

Test Code

LAB00459

Alias/See Also

LAB00459
Mayo TC: RBCME
Band 3
EMA Binding

CPT Codes
85557,88184,85060

Includes

Osmotic Fragility
Spherocytosis Interpretation
Band 3 Fluorescence Staining, RBC
Peripheral Blood Smear Review
Shipping Control Vial

Preferred Specimen

Patient:
Specimen Type: Whole blood and 2 Wright-stained Slides
Container/Tube: 2 vials of 4 mL lavender-top tubes

Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
2. Label clearly on outermost label normal control.

Patient Preparation
THIS TEST MUST HAVE A PATIENT CONTROL DRAWN. SEE "NORMAL SHIPPING CONTROL" ABOVE

Minimum Volume

4 mL Whole Blood EDTA, 2 Wright-stained slides, and a patient control as above

Instructions

Instructions
Patient:
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
2. Send specimen in original tube. Do not aliquot.
3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.
4. Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.
Additional Information: Patient's age and sex are required.

Normal Shipping Control:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 4 mL
Collection Instructions:
1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.
2. Label clearly on outermost label normal control.
3. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4 degrees C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.
4. Send specimen in original tube. Do not aliquot.
5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

Transport Container

Send specimen in original tube. Do not aliquot.

Transport Temperature

Refrigerated

Specimen Stability

Refrigerated: 72 hours

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis, clotted or frozen specimens.
No shipping control.

Methodology
Osmotic fragility and eosin-5-maleimide (EMA) binding (Band3) flow cytometry testing.

Report Available

7 days

Limitations

Test can only be drawn Monday-Wednesday
*Must draw a patient normal control!

Clinical Significance

Used for investigation of suspected red cell membrane disorders such as hereditary spherocytosis or hereditary pyropoikilocytosis

Performing Laboratory
Mayo Clinical Laboratories

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.