A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Vitamin B1 (Thiamine), Plasma/Serum, LC/MS/MS
Test Code90353
Alias/See Also
LAB125
CPT Codes
84425
Preferred Specimen
2 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light
Patient Preparation
Overnight fasting is required
Minimum Volume
1 mL
Other Acceptable Specimens
Frozen plasma foil wrapped (light-protected) • Frozen serum in an amber transport tube or foil wrapped (light-protected)
Instructions
Plasma: Collect blood in a lavender-top tube. Remove plasma within 24 hours of collection and transfer to a light protected pour off tube. If separation of cells can't be performed immediately after collection, keep the whole blood refrigerated and protected from light. The separation of cells must be completed within 24 hours of collection. Separate cells by centrifugation at 2-8° C for 8-10 minutes. Transfer plasma to amber tube to protect from light and freeze tube immediately. Alternately, neutral colored tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30° C.
Serum: Collect blood in plain red-top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.
Serum: Collect blood in plain red-top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil.
Transport Container
Amber transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 48 hours
Frozen: 30 days
Refrigerated: 48 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Not protected from light
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Tues-Sat
Report Available
3-4 days
Reference Range
Adult | 8-30 nmol/L |
Clinical Significance
Vitamin B1 deficiency is most often associated with alcoholism, chronic illness and following gastric by-pass surgery. Prolonged deficiency causes beriberi. Plasma vitamin B1 is useful in evaluating nutritional assessment and compliance, while whole blood vitamin B1 is useful in evaluating body stores.
Performing Laboratory
med fusion |
2501 South State Hwy 121, Suite 1100 |
Lewisville, TX 75067-8188 |