17-Hydroxyprogesterone, Neonatal/Infant

Test Code
17654

CPT Codes
83498

Preferred Specimen
0.5 mL serum collected in a red-top tube (no gel)

Minimum Volume
0.25 mL

Other Acceptable Specimens
Plasma collected in EDTA (lavender-top), or EDTA (royal blue-top), or sodium heparin (green top), or lithium heparin (green-top) tube

Instructions
Draw blood in a no-additive (red-top) tube. Separate serum after clotting. Do not submit glass tubes.

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum separator tubes (SST®)

Methodology
Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range
Pediatric Reference Ranges for 17-Hydroxyprogesterone, LC/MS/MS:
1-12 months: 11-170 ng/dL**
1-4 years: 4-115 ng/dL**
5-9 years: 90 ng/dL or less
10-13 years: 169 ng/dL or less
14-17 years: 16-283 ng/dL
Premature Infants (31-35 weeks)**: ≤ 360 ng/dL
Term Infants (3 days)**: ≤ 420 ng/dL
Tanner Stages:  
II-III Males: 12-130 ng/dL
II-III Females: 18-220 ng/dL
IV-V Males: 51-190 ng/dL
IV-V Females: 36-200 ng/dL
   

**Includes data from J Clin Endocrinol Metab. 1991;73:674-686; J Clin Endocrinol Metab. 1989; 69:1133-1136; and J Clin Endocrinol Metab. 1994; 78:226-270.

Clinical Significance
17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.