Tobramycin Peak

Test Code

TOBRAP

CPT Codes
80200

Preferred Specimen

Serum (gold top) tube

Minimum Volume

0.5 mL

Other Acceptable Specimens

Lithium Heparin Plasma (green top) tube

Instructions

To obtain a serum tobramycin concentration that best represents the peak tissue level, draw the sample one hour after an intramuscular injection, 30 minutes after ending a 30- minute intravenous infusion, or immediately after a 60-minute intravenous infusion. Collect a trough sample just before the next scheduled dose. When adjusting dosage, measure peak and trough levels during the same dosing intervals.

Transport Container

Serum (gold top) tube or Lithium Heparin Plasma (green top) tube

Transport Temperature

Room Temperature or Refrigerated

Specimen Stability

Room Temperature - 2 hours; Refrigerated - 7 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Quantity Not Sufficient, IV contamination

Methodology
Photometric

Setup Schedule

Daily upon receipt

Report Available

Upon completion of analysis

Limitations

Results for samples from patients receiving Amikacin, Dibekacin, Gentamicin, Kanamycin or Neomycin may be falsely elevated due to cross-reactivity in this assay (Amikacin, approximately 1%; Dibekacin, approximately 55%; Gentamicin, approximately 2%; Kanamycin, up to 23%).

Reference Range

No reference range given for peak Tobramycin..

Critical range: 24.0 ug/mL or higher

Clinical Significance

Tobramycin is used in the treatment of serious infections caused by susceptible strains of gram-negative microorganisms and particular gram-positive organisms that are resistant to less toxic antibiotics. Tobramycin is safe and effective only in a narrow range of concentrations for a given indication. Exposure to high tobramycin concentrations for a prolonged period may cause renal impairment or ototoxicity. Serum or plasma tobramycin measurements are used in the diagnosis and treatment of tobramycin overdose and in monitoring levels of tobramycin to ensure appropriate therapy.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.